AKT1 and melanoma: These unique pathways in the 48 h group or 72 h group indicated the time-dependent response of SK-MEL-2 cells to polymyxin B. The protein-protein interaction analysis showed complex interaction maps and the pathway enrichment also showed PI3K/Akt signaling pathway, lysosome, oxidative phosphorylation, and ribosome were the most interacted proteins in 48 h group, while PI3K/Akt signaling pathway, complement, and coagulation cascades and melanoma pathways were in 72 h group.