Data generated from surrogate DCs used in methods such as h-CLAT (THP1 line), U-SENS (UP37 line), IL-8 Luc (validated, OECD TG 442E), and GARD (under validation, for inclusion in OECD TG 442E) [MUTZ-3 and SenzaCells (ATCC Depository PTA-123875)] address the same AOP (skin sensitization) event, namely DC activation. This evidence concerns the gene CXCL8 and skin sensitization.