Given that Stx2-positive microvesicles were reported to be taken up in the murine glomerular endothelium in an EHEC infection model (Stahl et al., 2015), and that mouse glomerular endothelial cells lack Gb3 expression (Psotka et al., 2009), further work provided evidence for microvesicle-mediated Stx2 uptake in cells bearing or lacking endogenous Gb3 (CHO, HeLa or DLD-1). Here, STX2 is linked to infection.