The bulk RNA-Seq data simulated by totaling whole cells in the dataset showed that with a higher infection rate in the Large dataset [~20% infection which is equivalent to the previous study (Seizova et al., 2021)], upregulation of the interferon response pathways was higher than that in the Deep dataset, as indicated by the CPM-normalized STAT1 levels (597, 258, and 155 in Large dataset, Deep dataset, and mock infected culture control, respectively; Data Set S1, Sheet 1). Here, STAT1 is linked to infection.