KM survival analysis showed that higher CHPF cg03176520 site methylation was correlated with better overall survival (OS) and progression-free survival (PFS) (Figures 2C, D) In addition, Breast cancer patients with both cg03176520 site hypermethylation and low CHPF gene expression had significantly increased overall survival contrasted to hypermethylation combined with high CHPF gene expression group (Figure 2E). This evidence concerns the gene CHPF and breast cancer.