To determine the functions of SLC16A1-AS1 in BC cells, SLC16A1-AS1 was successfully overexpressed after transfection of pcDNA3.1 plasmid containing the SLC16A1-AS1 sequence in MCF7 and MDA-MB-231 cells, which was confirmed by qRT-PCR assay (Figure 2A). Here, SLC16A1 is linked to breast cancer.