We showed that overexpression of miR-181a-5p decreased cell proliferation and suppressed MMP9 expression by downregulation of the MAPK pathway Moreover, the presence of miR-181a-5p in clinical samples and EVs isolated from cellular supernatants as well as patient sera justifies further studies to reveal a potential role of miR-181a-5p in GBM diagnosis and progression. Here, MMP9 is linked to glioblastoma.