The relative expression level of NUS1 in these cell lines is opposite to that of miR-184-5p in same cell lines as is shown in Figure 1D. Next, to explore the effect of NUS1 in progression of ccRCC, we transfected two siRNAs specific for NUS1 (siNUS1-1 and siNUS1-2) and pcDNA3.1-NUS1 (NUS1 overexpression plasmid, NUS1-OE) into A-498 and 786-O cells. The gene discussed is NUS1; the disease is nonpapillary renal cell carcinoma.