To validate the different mRNA and protein expression levels of the 9 prognostic genes (ABCC5, BCL10, FDPS, ITGB1BP1, MSH2, PABPC1, PRDX1, SLC25A19, and YWHAH) between HCC and adjacent non-tumorous tissues, qRT-PCR and IHC were performed. This evidence concerns the gene ITGB1BP1 and hepatocellular carcinoma.