NRAS and melanoma: By western blot assay, we detected high levels of ERα in the primary wild type melanoma lines Me1007 and Mel501 and in the BRAF mutated WM983A, it was weakly visible in Me1402/R, a mutated BRAF recurrence of primary melanoma, and barely detectable in NRAS-mutated metastatic Me665/1 and SK-Mel 30 cell lines (Fig. 2A).