Based on the PICRUSt2 analysis, dietary E. faecium may modulate the cysteine and methionine metabolism, Tryptophan metabolism, the NOD-like receptor signaling pathway, and PI3K-Akt signaling pathway and ultimately inhibit salmonella infection by improving the intestinal homeostasis, which was similar to a previous result to some extent. The gene discussed is AKT1; the disease is Salmonella Infections.