Among the 41 related genes, there were 8 depression-related genes (Clasp1, Klf4, Oxtr, Adgra2, Sgms1, Kcna1, Zc3h12c) (Table 1) with which genetic validation was performed in the double-stress group with low expression of hypermethylation and LPM570065 group with high expression of hypomethylation by RT-PCR and pyrophosphate sequencing. This evidence concerns the gene ADGRA2 and major depressive disorder.