As displayed in Table 4, binary logistic regression analysis showed that subjects in the highest tertile of serum RCAN2 levels had a 2.114-fold higher risk of NAFLD than those in the lowest tertile levels (OR = 3.114, 95% CI = 2.141–4.531, p = 0.000). This evidence concerns the gene RCAN2 and metabolic dysfunction-associated steatotic liver disease.