In the NAFLD group, serum RCAN2 levels were positively associated with SBP (r = 0.105), GLO (r = 0.119), ALP (r = 0.127), UA (r = 0.208), TC (r = 0.266), TG (r = 0.458), and FLI (r = 0.198) but negatively associated with A/G (r = −0.110) and HDL-C (r = −0.108) (all p < 0.05). Here, RCAN2 is linked to metabolic dysfunction-associated steatotic liver disease.