This study investigated the effect of Perftoran® on ICG/PDT efficacy in presence and absence of Perftoran®via evaluation of phototoxicity by MTT; hypoxia estimation by pimonidazole, HIF-1α/β by ELISA, and 17 miRNAs (tumor suppressors, oncomiRs, and hypoxamiRs) were analyzed by qPCR. This evidence concerns the gene HIF1A and neoplasm.