Two different portal hypertensive animal models, bile duct ligation (BDL) mice as a portal hypertensive model resulting from cirrhosis and partial portal vein ligation (PPVL) mice as an idiopathic portal hypertensive model without hepatic dysfunction (Abraldes et al., 2006; Geerts et al., 2008; Bosch and Iwakiri, 2018), were prepared to analyze the functional expression of TMEM16A channels in PVSMCs. This evidence concerns the gene ANO1 and liver disorder.