To test whether the roles of IL1B and CCL2 in generating SNHL are mediated by regulating ROS production, we examined ROS levels in HEI-OC1 cells with different expressions of IL1B and CCL2. The ROS levels were indeed significantly higher in HEI-OC1 cells treated with D-gal than without any treatments (Figures 4I,J). The gene discussed is CCL2; the disease is sensorineural hearing loss disorder.