For 2-h transient MCAO in mice, NLRP3 and caspase-1 expression are significantly upregulated at 12 h, peaking at 24 h, and remaining elevated for more than 48 h in a time-dependent manner (Yang et al., 2014); while for permanent MCAO in mice, NLRP3 and caspase-1 expression are detected in ischemic penumbra within 24 h, peaking at 3 days and remaining elevated for 7 days after stroke (Barrington et al., 2017; Liang et al., 2020). Here, NLRP3 is linked to stroke disorder.