Our prior study has confirmed the association between Nestin and ESCC, and the results of that study lead us to 3 conclusions: 1) Nestin can be expressed in ESCC tissues and cell lines; 2) Nestin-positive ESCC is associated with a poor prognosis; 3) A positive correlation between Nestin phenotype and tumor cell proliferation indexes (Ki67 and PCNA) indicated that Nestin may be involved in the malignant proliferation of ESCC. Here, PCNA is linked to neoplasm.