In the challenge experiment, a slight increase in Salmonella shedding was observed in response to pig transfer to the grower houses, regrouping and experimental re-infection with S. Typhimurium.  ANE supplementation had no effect on Salmonella counts; nevertheless, it reduced Enterobacteriaceae counts, as well as the expression of the inflammatory IL22 and TGFB1 which are associated with colonisation and persistent Salmonella infection. This evidence concerns the gene IL22 and Salmonella Infections.