In summary, based on the above open-access database and our experimental data, we revealed that not only NEK2 was highly expressed in breast cancer and correlated with prognosis, but also the expression level of NEK6, NEK8, and NEK11 were significantly correlated with molecular subtypes and prognosis of breast cancer, which are potential prognostic markers and new therapeutic targets. This evidence concerns the gene NEK2 and breast carcinoma.