Within the allergic diseases and moderate-to-severe asthma gene-sets, FLG was nominally significant in the loss-of-function burden test (P = 0.024, OR = 1.473), and eight additional genes passed nominal significance in the high or moderate (PolyPhen > 0.5) impact burden test (RERE, IQCB1, PPP2R3C, PITPNM2, DYNAP, TSLP, EAF2, RASA2) (Supplemental Tables S9 and S10). The gene discussed is RASA2; the disease is allergic disease.