A volcano plot revealed many differentially expressed genes (DEG) between TAb2 vs. TCh3 tumor cells, such as colony stimulating factor 1 (CSF1) and MMP2 upregulated in TAb2 tumors (Fig. 3A), suggesting that the enrichment of specific genes may explain the heterogeneous TME and differential ICI responses between TAb2 and TCh3 tumors. The gene discussed is CSF1; the disease is neoplasm.