However, anti-PD-L1 treatment resulted in upregulation or downregulation of numerous genes in CD45+ tumor-infiltrating immune cells in TCh3 tumors, including Cd3d, Cd3e, Cd3g, Cd8a, Cd8b1, Nkg7, Ccl5, Ets1, Icos, Cxcr6, Pdcd1, Lag3, Prf1, and Gzmb (Fig. 7E, right). This evidence concerns the gene ICOS and neoplasm.