In a comparison of a benign human thyroid cell line to thyroid cancer cell lines, 25–50 μM NaHS increased CBS, sulfide:quinone oxidoreductase, p-PI3K, p-AKT, p-mTOR, H-RAS, p-RAF, p-MEK1/2, and p-ERK1/2, and rhodanese protein expression, while enhancing cell proliferation, migration, and viability in the cancer, but not benign cell lines. Here, CBS is linked to thyroid cancer.