To note, it was observed that Cx43 showed perinuclear localization in CuFi-5 cells (F508del homozygous), while Cx26 localization was unaffected, and both Cxs were at the correct position in non-CF NuLi cells [31], further demonstrating a defect in cell-to-cell contacts in CF airway epithelia. Here, GJA1 is linked to cystic fibrosis.