In contrast to the CD8+ T cells marker that localized to specific tumor areas, evenly distributed CD4 staining was detected (Figure 2C,D) in both MNG and GBM samples indicating the presence of CD4+ helper T cells throughout both tumor tissues that are more likely to be immune suppressive in nature (regulatory T cells (Treg)) due to the elevated levels of FOXP3. Here, FOXP3 is linked to glioblastoma.