To determine whether MLCK210 is required for integrin α4-mediated adhesion, Mlck210−/− and WT murine peripheral blood CD11b+ myeloid cells were incubated with basal medium or medium containing IL-1β, IL-6, SDF-1α, TNFα, VEGF-A, or Lewis lung carcinoma cell conditioned medium (TCM). The gene discussed is TNF; the disease is Carcinoma, Lewis Lung.