We also classified the spots of CRC patient #7 and #8 into six clusters, i.e., FAP+ fibroblasts/SPP1+ macrophages, malignant epithelial cells, MSC cells, myofibroblast cells, immune cells, and unknown (Fig. 6d–f, and Supplementary Fig. 9a–c), classified the spots of CRC patient #9 into 8 clusters, FAP+ fibroblasts/SPP1+ macrophages, malignant epithelial cells, epithelial cells, MSCs, endothelial cells, myofibroblasts, immune cells, and unknown (Supplementary Fig. 9d–f). The gene discussed is SPP1; the disease is colorectal carcinoma.