SPP1 and colorectal carcinoma: Based on the flow cytometric analysis of myeloid cell subset markers CD14, CD206 (encoded by MRC1), CD209, and CD13 (encoded by ANPEP) (Fig. 4f), we further documented that SPP1+ macrophages were significantly increased in CRC compared with non-malignant colon tissue while THBS1+ macrophages were non-significant change (Fig. 4g, Supplementary Fig. 6e, f).