The combination of scRNA-seq, ST, and multiple ion beam imaging revealed the main locations of tumor-specific keratinocytes (TSK) populations at the leading edge of human cutaneous squamous cell carcinoma.106 TSK cells recruit specific cell types like Treg cells to cancer sites by integrating ligand-receptor networks as the hub of cell-cell communication and express chemoattractive factor genes, while CD8 T cells and neutrophils are absent in the tumor microenvironment (Fig. 4d). This evidence concerns the gene CD8A and neoplasm.