Notably, the tumor purity-adjusted DEPTH2 scores showed significant positive correlations with TMB, CNA, and HRD in 5, 11, and 12 cancer types, respectively (P < 0.05) (Additional file 3: Fig. S2A); they correlated inversely with the enrichment scores of CD8+ T cells, NK cells, immune cytolytic activity, and IFN response in 14, 9, 10, and 13 cancer types, respectively (P < 0.05) (Additional file 3: Fig. S2B); they correlated positively with stemness scores in 11 cancer types (Additional file 3: Fig. S2C). Here, CD8A is linked to cancer.