The enrichment scores of CD8+ T cells had a significant negative correlation with ITH scores by MATH, PhyloWGS, ABSOLUTE, DEPTH, and tITH in 10, 10, 9, 21, and 20 cancer types, respectively, compared to DEPTH2 in 19 cancer types (P < 0.05) (Fig. 9C). This evidence concerns the gene CD8A and cancer.