CD8A and cancer: We found that the enrichment levels of these immune signatures had significant inverse correlations with DEPTH2 scores in pan-cancer (CD8+ T cells: ρ = -0.34, P = 1.36 × 10–293; NK cells: ρ = − 0.10, P = 3.28 × 10–26; immune cytolytic activity: ρ = − 0.16, P = 4.11 × 10–63; IFN response: ρ = − 0.27, P = 2.86 × 10–181) and in 19, 10, 11, and 17 cancer types, respectively (P < 0.05) (Fig. 4A).