Besides,the accuracy 1/MCP-1 mRNA for overt DN detection was 0.66 (95%CI: 0.55–0.77, p = 0.007), and 1/MCP-1 mRNA level ≥ 5.89 as the best cutoff value resulted in 57.1% as sensitivity, 74.2% as specificity, 55.5% as PPV, and 75.3% as NPV for discrimination of overt DN from DM. The gene discussed is CCL2; the disease is diabetes mellitus.