PFKFB3 and neoplasm: To confirm the role of aerobic glycolysis in regulating CA12 expression in tumor-exposed monocytes and macrophages, CD14+ cells were purified from the peripheral blood of healthy donors and treated with HepG2 TSN in the presence or absence of the glycolysis inhibitor 2-deoxy-d-glucose (2DG) or the PFKFB3 (a key glycolytic enzyme) inhibitor 3PO.