To investigate the second role of ZBP1, namely induction of necroptosis, we knocked down ZBP1 in B16F10 tumor cells (Fig. 5D) and evaluated their cell cycle distribution and cellular stress levels, as monitored by quantitative image-based cytometry (QIBC) and γH2AX induction (Fig. 5E). This evidence concerns the gene ZBP1 and neoplasm.