To further verify the interplay between γδ T and CD4+ T cells in vivo, we treated γδ T reconstituted TCRδ-/- mice with α-CD4 depleting antibody, the results showed that α-CD4 treatment prevented the development of liver fibrosis compared to the control group (Fig. 6d–j), corroborating that IL-17-producing CD4+ T cells play a critical role in the pathogenesis of liver fibrosis, and Th17 differentiation was suppressed by IFN-γ producing Vγ4 cells. This evidence concerns the gene CD4 and Hepatic fibrosis.