TAICs including pan-T cells (CD3+), T-helper cells (CD4+), regulatory T cells (T-regs (FOXP3+), T-cytotoxic cells (CD8 +GZMB+), B cells (CD20+), natural killer cells (CD57+), and macrophages (CD68+) were detected by IHC on surgical GC samples without or after NAC chemotherapy, respectively. Here, CD4 is linked to gastric cancer.