To further compare the NAC effect on immune microenvironment, a panel of multiplex immunofluorescence staining (CD3, CD4, CD8, Foxp3, and PDL1; figure 2A) were performed in paired samples before and after chemotherapy in another independent cohort of 30 patients with GC including 15 response cases and 15 non-response cases (online supplemental table S5). The gene discussed is CD4; the disease is gastric cancer.