Overall, TCR Vβ segments identified in the activated T cell populations were also evident in the primary tumor (n=5 for CD4+) and, more commonly, the metastasis (n=5 for CD8+ and n=12 for CD4+) consistent with our initial findings for KIAA0408-expanded bulk CD8+ and CD4+ T cell populations; this included two TCRs that were observed in the previous KIAA0408 MUT dataset and the metastasis, n=1 from CD8+CD137+ T cells (sample 1) and n=1 for CD8+CD137+ and/or CD69/PD1+ T cells (sample 3). This evidence concerns the gene CD8A and neoplasm.