We performed TCR-seq on whole tumor (snap frozen tissue): (1) primary tumor and (2) metastasis, and on PBMC from the blood, on bulk sorted CD8+ and CD4+ T cells: (3) unstimulated PBMC directly ex vivo, and on PBMC expanded with (4) positive control viral peptides and (5) an independent assessment of PBMC expanded with MUT (reactive) synthetic peptides and corresponding WT synthetic peptides, with the exception of KIAA0408 WT, where there were not enough cells remaining after ELISpot for TCR-seq analysis. The gene discussed is CD4; the disease is neoplasm.