Then, WGCNA, COX univariate regression analysis, random forest algorithm and multiple combinations were used to construct this nine-gene signature (including RNF144A, STC1, P4HA1, FMNL3, ANO1, BASP1, MME, PLEKHG2 and DKK1) with prognostic value for HNSCC patients. Here, ANO1 is linked to head and neck squamous cell carcinoma.