Through Western blotting, we observed that an overexpression of miR-509-3p within the CRC cell lines caused a significant decrease in the expression of epithelial cadherin (E-cadherin) with a concurrent increase in the expression of Ras Homolog Family Member A (RhoA) protein (Figure 3C1-C3), relative to the negative control. This evidence concerns the gene RHOA and colorectal carcinoma.