CD14 and Chagas disease: To determine whether certain monocyte subsets were particularly expressed in Chagas disease patients, according to the disease stage, the monocyte population in whole PBMC from HI, Asy and CHD patients was characterized by flow cytometry analysis (FACS), according to the expression of CD14 and CD16, as classical (CD14high CD16neg), intermediate (CD14high CD16pos) and non-classical (CD14low CD16pos) (Figure 3A).