Logistic regression analysis on the results obtained for the training cohort identified three promising combinations of markers to be used to rule-out infection: HBHA- and ESAT-6-induced IFN-γ, HBHA- and ESAT-6-induced TNF-α, HBHA-induced IL-2 and ESAT-6-induced TNF-α. The gene discussed is IFNG; the disease is infection.