Interestingly, challenging these neurons with human AD brainhomogenate containing Aβ causes a decrease in Kv3.4 expression alongside anincrease in curvature of MAP2 positive neurites compared to AD brainhomogenate immunodepleted to remove Aβ (Figure 5, ANOVA of linearmixed-effects model of data transformed with the formula(Tortuosity − 1)1/7 to fit assumptions of model:F[1,6944] = 15.21, p < 0.0001). This evidence concerns the gene KCNC4 and Alzheimer disease.