In thissame study, protein level measured by western blot was also increased in ADcompared to control frontal cortex, and Kv3.4 staining withimmunohistochemistry was observed in a punctate pattern in all cases, withsome accumulation around plaques in AD samples (Angulo et al., 2004).Furthermore, increased Kv3.4 protein levels were observed in three Tg2576transgenic mice (which develop amyloid plaques) compared to three wild-type(WT) mice (Angulo etal., 2004). The gene discussed is KCNC4; the disease is Alzheimer disease.