The expression of UNC5D protein was significantly upregulated and the expression of E2F3 was also increased in Y79 and WERI-Rb-1 retinoblastoma cell lines as compared to hTERT-RPE cells whereas the expression of ESR1 protein was downregulated in the above two retinoblastoma cell lines. The gene discussed is UNC5D; the disease is retinoblastoma.