SPRED1 and acute myeloid leukemia: Meanwhile, SPRED1 hypermethylating cell lines were screened by bisulfite sequencing PCR (BSP) method and the possible relationship between aberrant SPRED1 methylation and leukemogenesis, which was confirmed by 5-aza-2′-deoxycytidine (5-AZA) and overexpressed lentivirus transfection assays in AML cell lines.