CTU1 and neoplasm: Instead, the promoter region of CTU1 gene was detected to be unmethylated in all CR tumor samples (40 CR tumor samples, 100% of cases), while in 27 TR tumors samples, it was hemimethylated; in 2 TR tumor samples, it was unmethylated and only in 1 TR tumor sample that it was methylated (90%, 6.6%, and 3.4% of cases, respectively) (Figure 4B).