We detected the expression of MMP-1 in situ and metastatic tumors of breast cancer xenograft in mice, and we found that MMP-1 decreased by 44.23% and 47.23% in FLNA/KO-1 and FLNA/KO-2 groups, respectively (Figures 5D, E). This evidence concerns the gene MMP1 and metastatic neoplasm.