ISG15 and endometritis: A total of 14 predicted upstream regulators were identified only in healthy cows (P < 0.05; Fig 5), while five unique predicted upstream regulators were identified only in cows after uterine infection, including the inhibition of ISG15 and AIRE, and activation of DUSP1, NFKBIA, and PF4 (P < 0.05; Fig 5).