HMGB1 and ischemic stroke: Consistent with a role for platelet-HMGB1-induced NET formation in ischemic stroke, thrombocytopenic mice were protected from brain injury (45.3 ± 20.3 mm3 vs. 88.2 ± 35.2 mm3; Figure 5, C and D) and had improved neurological function (Supplemental Figure 8, B and C).