We developed a reversal protocol in which ISM-Cre and S-RhoBTB1 littermates received either vehicle or Ang II infusion via osmotic minipump (490 ng/kg/min for 6 weeks), followed by 5 consecutive daily Tx injections 2 weeks after Ang II–induced hypertension was established (Figure 1B). This evidence concerns the gene AGT and hypertensive disorder.