We examined the possibility that the prevalence of somatic TSC2/TP53 mutations in skin biopsies might be associated with different clinical characteristics, including FAF grade (Facial Angiofibroma Severity Index score; ref. 45), age at biopsy, pigmentation, latitude at which donors had lived for most of their lives, and degree of sun exposure. This evidence concerns the gene TSC2 and Angiofibroma.