These samples were derived from individuals with TSC with low-level systemic TSC2 mosaicism detected previously in various fluids and tissues, including blood, saliva, semen, normal skin, and TSC skin lesions, available from each patient (including 19 FAF, 10 TSC normal skin, 13 blood, 1 semen, and 1 UF samples) or heterozygous TSC2 mutations (3 TSC nipple angiofibroma [AF] samples) (Table 1 and Supplemental Table 2). This evidence concerns the gene TSC1 and tuberous sclerosis.