An MHPA assay for TP53 was also generated, using 12 primer pairs to amplify similar short segments (90–126 nt; queried sequence, Supplemental Table 1.2) of the exons and exon-intron junctions of TP53, covering all hotspot regions and 93% of somatic nonsynonymous TP53 variants, identified previously in normal keratinocytes and skin cancers (Supplemental Table 1.2 and Supplemental Figure 1). Here, TP53 is linked to skin cancer.