Similarly, Western blot assay showed an increased expression of Bcl-2 and reduced levels of Bax and cleaved caspase 3 after induction of autophagy whereas 3 M treatment caused the opposite phenomenon inversely, which was in line with previous results that lncRNA HAGLROS facilitated cell proliferation, suppressed apoptosis and accelerated autophagy in hepatocellular carcinoma cells through the regulation of miR-5095/ATG12 axis [18]. This evidence concerns the gene ATG12 and hepatocellular carcinoma.