JUN and acute myeloid leukemia: Additionally, to examine the involvement of miR-216b in the anticancer effect of DD and apoptosis, we performed qRT-PCR of miR-216b, together with Western blotting of p-c-Jun in AML cells, and we observed that miR-216b level was highly increased in THP-1 and moderately in U937 cells (Figures 6A–D).